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Deciphering gene function. Understanding disease biology.

RNA silencing with no transfection reagent. From difficult-to-transfect cells to in vivo applications.

Species

Nothing is chosen: a page and an order name one species.

A gene, a microRNA or a long non-coding RNA, in human, mouse or rat. Order to your own sequence

Product selection guide Talk to a scientist
Inside a cell, a self-delivering antisense oligonucleotide, a violet gapmer with a pale central gap between its two wings, is paired along its length with a blue-teal messenger RNA in the cytoplasm, and RNase H1, a translucent globular enzyme, sits on the gap under a warm glow: the messenger RNA is cut at the enzyme, its capped start on one side and its beaded tail on the other, while the oligonucleotide stays whole. Three more gapmers drift in the dark medium just outside the cell membrane with no transfection reagent; the membrane takes one in through a pit and a vesicle, one crosses a nuclear pore into the nucleus at the top right, and a mitochondrion lies below the pair.

Sequence design

The AUMsilence platform

Every sequence is designed by AUM against the target the customer names: a gene, a microRNA, a long non-coding RNA or a viral genome. The same design is made in three forms: AUMsilence sdASO, which is self-delivering, and AUMsilence toASO and AUMsiRNA, which are delivered with a transfection reagent.

YOUR TARGETTHE ENGINETHE PRODUCTTHE CELLmRNAMicroRNALong non-coding RNAViral RNAAUMsilence™ platform01Reads the transcript02Scores every window03Builds them with its chemistryAUMsilence™ sdASO™self-delivering, no reagentAUMsilence™ toASO™with a transfection reagentAUMsiRNA™with a transfection reagenttaken up by endocytosis, no transfection reagentarrives inside a transfection reagent
One engine designs every product. AUMsilence sdASO is taken up by endocytosis with no transfection reagent. AUMsilence toASO and AUMsiRNA arrive inside a transfection reagent, the lipid sphere on the cell membrane.
How a sequence is designedThe platform

Self-delivering

No lipid-based transfection reagent, electroporation, or viral vector required.

Key advantages over RNAi and gene-editing

The AUMsilence platform against siRNA, shRNA and CRISPR-based methods

  • Transfection-free delivery

    AUMsilence sdASOs are self-delivering. They are added directly to cell culture or injected in vivo, with no lipid transfection reagent, electroporation, or viral vector.

  • Broad compatibility

    AUMsilence sdASOs are used in cell lines, primary patient cells and animal models. Knockdown is target and cell-type dependent.

  • High specificity and low off-target effects

    AUMsilence sdASOs are scored for specificity against the transcriptome before synthesis. Off-target effects are sequence-dependent and are checked with a non-targeting control.

  • No cytotoxicity at the optimized working concentration

    The working concentration is determined by dose titration for each cell type and target. At this concentration AUMsilence sdASOs do not cause cytotoxicity. A reduction in viability reflects the knockdown rather than the chemistry, and the non-targeting control shows whether the knockdown is on target. No transfection reagent, electroporation, or viral vector is required, so the cytotoxicity associated with these delivery methods is eliminated.

  • Scalable and reversible

    AUMsilence sdASOs are added to the culture medium. No plasmid is constructed and no cell line is engineered, which a CRISPR or shRNA workflow requires. Knockdown by AUMsilence sdASOs is transient and tunable: stop treatment and gene expression will return.

  • In-depth control and versatility

    Target mRNA with AUMsilence sdASOs, inhibit microRNA with AUMantagomir sdASOs, or silence long non-coding RNA with AUMlnc sdASOs, for functional genomics, target discovery, and validation.

See the advantages

The research desk

Learn about your target

  • Summary
  • Transcripts
  • Expression
  • Protein
  • Interactions
  • Pathways
  • Disease
  • Variants
  • Constraint
  • Orthologs
  • MicroRNAs
  • Long non-coding RNAs
  • Literature
  • Explore your target

Each panel names the public database it was read from.

or try:

Drug discovery

Use at each stage of drug discovery

From target identification through preclinical development

  1. 01

    Target identification

    Screen genes with sdASO libraries for phenotypic effects

  2. 02

    Target validation

    Confirm target role by silencing

  3. 03

    Drug discovery

    Evaluate antisense oligonucleotides (ASOs) as candidates in disease models

  4. 04

    Preclinical development

    In vivo efficacy with self-delivering ASOs, production at scale for preclinical studies

  • Self-delivering technology

    No transfection reagent, electroporation, or viral vector. Add to cell culture or inject in vivo.

  • Compatible cell types

    Works in primary cells, neurons, immune cells, stem cells, 3D cultures, organoids, and animal models.

  • Targeted RNA classes

    Target mRNA with AUMsilence sdASO, microRNA with AUMantagomir sdASO, long non-coding RNA with AUMlnc sdASO, and viral RNA with AUMsilence V+ sdASO.

One platform, every stage of drug discovery: from target ID to preclinical studies

The products

RNA silencing products

Four families: the AUM products and the conventional chemistries

Self-delivering antisense oligonucleotides (sdASO)

Self-delivering antisense oligonucleotides designed by the AUMsilence platform and taken into the cell with no transfection reagent.

  • AUMsilence sdASO

    RNase H1 on a gapmer paired with a messenger RNA inside a cell: the messenger RNA is cut at the enzyme with a gap, and the gapmer stays whole.

    mRNA knockdown

    Self-delivering antisense oligonucleotides for mRNA silencing in cell lines and hard-to-transfect primary cells, with 70-95% knockdown.

    Learn more Order

  • AUMantagomir sdASO

    A short microRNA paired end to end with one uniform oligonucleotide strand under a warm glow, nothing cut, and an empty Argonaute protein beside the pair.

    miRNA inhibition oligos

    Tailored for microRNA inhibition, allowing researchers to block specific miRNAs and reveal their biological functions and their part in disease.

    Learn more Order

  • AUMmimic sdASO

    One uniform oligonucleotide strand held inside an Argonaute protein that sits on a messenger RNA near its beaded tail; the message runs through unbroken.

    miRNA mimic oligos

    Self-delivering antisense oligonucleotides that supply a chosen mature microRNA: the counterpart of AUMantagomir sdASOs, which inhibit one.

    Learn more Order

  • AUMlnc sdASO

    Inside the nucleus, among chromatin, RNase H1 on a gapmer paired with a long non-coding RNA, which is cut at the enzyme; the gapmer stays whole.

    lncRNA knockdown oligos

    Specifically optimized to bind and degrade lncRNA transcripts, including nuclear-retained RNAs beyond the reach of traditional RNAi.

    Learn more Order

  • AUMblock sdASO

    One uniform oligonucleotide strand paired at the capped start of a messenger RNA, with the two parts of a ribosome held apart above and below it; nothing is cut.

    Blocker oligos

    Steric-blocking self-delivering antisense oligonucleotides that bind target RNAs and modulate their function without recruiting RNase H.

    Learn more Order

  • AUMsplice sdASO

    The spliceosome holding two exons of a pre-messenger RNA together while the exon between them loops out below, one uniform oligonucleotide strand paired on the loop; nothing is cut.

    Splice modulation and exon skipping ASOs

    Self-delivering antisense oligonucleotides designed to bind pre-mRNA and redirect splicing, typically by causing a specific exon to be skipped during mRNA processing.

    Learn more Order

  • AUMsilence V+ sdASO

    RNase H1 on a gapmer paired with a viral RNA, which is cut at the enzyme with a gap; the gapmer stays whole.

    Viral RNA knockdown oligos

    Self-delivering antisense oligonucleotides against viral RNA, for virology research and antiviral target discovery.

    Learn more Order
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Where it works

Knockdown in difficult-to-transfect cells

70-95% knockdown

  • Neurons and glia

    Cortical, hippocampal, motor and dopaminergic neurons; iPSC-derived neurons; astrocytes, microglia, oligodendrocytes

  • Immune cells

    T cells, B cells, NK cells, macrophages, dendritic cells, CAR-T and CAR-NK cells, TILs

  • Stem and progenitor cells

    Human and mouse ESCs, iPSCs, HSCs, CD34+ HSPCs, MSCs, intestinal stem cells

  • 3D models and organoids

    Tumor spheroids, patient-derived tumoroids, brain, intestinal, kidney and liver organoids, organ-on-chip

  • Cancer models

    Solid tumors, hematologic malignancies, patient-derived neurospheres and tumoroids, PDX-derived cultures

  • In vivo, no carrier required

    12 delivery routes: intravenous, intraperitoneal, intrathecal, stereotaxic, intranasal, intravitreal and more

Every compatible cell type, 3D model, organoid and delivery route, with the in vitro workflow and the administration sites.

See where it works

Research applications

Applications by research stage and research field

  1. 01

    Basic fundamental research

    • Gene function studies
    • Pathway dissection
    • RNA biology discovery
    • Mechanism of action
  2. 02

    Translational research

    • Target validation
    • Biomarker discovery
    • Disease modeling
    • Preclinical studies
  3. 03

    Preclinical research

    • Candidate development
    • Patient-derived models
    • Preclinical candidate testing
    • Scale-up for preclinical work

Research fields

  • Cancer research

    Target oncogenes and tumor suppressors in solid tumors, liquid cancers, and metastatic models

  • Neurodegenerative diseases

    Study Alzheimer's, Parkinson's, ALS, and Huntington's disease mechanisms in neurons and glial cells

  • Viral infections

    Investigate host-pathogen interactions and antiviral targets for COVID-19, HIV, influenza, and emerging viruses

  • Immunology

    Modulate immune responses in T cells, B cells, macrophages, and dendritic cells for target discovery

  • Stem cell biology

    Control cell fate decisions, iPSC differentiation, and developmental pathways with reversible gene silencing

  • Gene regulation

    Dissect miRNA networks, lncRNA functions, and regulatory cascades

AUMsilence sdASO technology supports every stage: from initial gene discovery to preclinical development.

Products

The three product types

Self-delivering, transfection-optimized, and siRNA

  • Self-deliveringNo reagent

    AUMsilence sdASO

    In cell culture and in vivo

    Self-delivering antisense oligonucleotides. Add to cells or inject in vivo: no carriers needed.

    • No transfection required
    • 70-95% knockdown
    • Works in primary cells
    • In vivo ready
    Explore AUMsilence sdASO
  • siRNAWith a reagent

    AUMsiRNA

    Singly or as a pool against one target

    Chemically modified siRNA, silencing through the RNAi pathway with a transfection reagent.

    • Enhanced stability
    • Reduced off-targets
    • Optimized design
    • Proven RNAi pathway
    Explore AUMsiRNA
  • Transfection-optimizedWith a reagent

    AUMsilence toASO

    The same designs at a lower price, delivered with a transfection reagent

    Antisense oligonucleotides optimized for transfection. Used for pilot and high-throughput experiments.

    • Cost-effective option
    • Standard transfection
    • Reliable knockdown
    • Bulk synthesis by quotation
    Explore AUMsilence toASO

Design is done by AUM after the order, as part of the price.

Why labs choose AUM BioTech

  • Reproducibility

    Consistent knockdown across experiments with minimal batch-to-batch variation

  • No reagent to optimize

    Delivery needs no transfection reagent, so none has to be optimized for the cell type

  • No equipment to buy

    No electroporator and no transfection reagent to buy

The table compares the three on delivery, applications and price.

Compare the products

Evidence

See the publications

Published in scientific journals

  • Nature
  • Science
  • Circulation
  • Nature Immunology
  • Nature Genetics
  • Blood
  • Cellular and Molecular Immunology
  • Journal of Extracellular Vesicles
  • Cell Stem Cell
  • Nature Communications
  • Science Immunology
  • Science Translational Medicine
  • Science Advances
  • Neuro-Oncology
  • Acta Neuropathologica
  • PNAS
  • The EMBO Journal
  • Cell Reports
  • Blood Advances
  • Aging Cell
  • Frontiers in Immunology

Support

Support before and after the order

Researchers worldwide publish with AUMsilence sdASO.

Explore our product pages, or contact our scientific team for a consultation on the target and the cell type.

Consultation and protocol support

A scientist reviews the target and the protocol

  • Consultation at no cost
  • Custom protocol development
  • In vivo optimization support

Works with mRNA, microRNA and long non-coding RNA targets

Talk to a scientist

Stay updated

New protocols and published studies, by email

  • Peer-reviewed research highlights
  • Protocol updates