| The basics |
| Not Required |
Transfection Reagents |
Required |
| AUMsilence oligo technology allows very high specificity for the target RNA |
Specificity |
siRNA grade specificity |
| None |
RISC associated Off target effects |
Yes |
| Easy and convenient single step process |
Transition from basic cell lines to primary cells to animal models |
Require extensive optimization and use of delivery reagents |
| Ease of use in different model systems |
| Easy to use. No transfection reagents required. Mix AUMsilence oligos with the cells |
Basic Cell lines |
Need transfection reagents. Transfection reagent based optimization required |
| Easy to use. No transfection reagents required. Mix AUMsilence ASO oligos with the cells |
Primary cells and difficult to transfect cells |
Need transfection reagents that kills the sensitive cells and sometimes alter the biology |
| Can work in fish models (example zebrafish) |
Fish models |
Not recommended for fish models |
| Key Attributes |
| Non-toxic |
Toxicity |
Can be toxic |
| Resistant to degradation by serum and cellular nucleases |
Stability |
Moderate stability |
| Minimal resources needed and easy to optimize |
Resources and time saving |
Require additional reagents and machines. Time consuming optimization |
| Up to 80% more cost effective in terms of time and money. |
Cost saving |
Expensive to use |