What is the difference between AUMsilence and siRNA?

AUMsilence uses a single-stranded antisense oligonucleotide mechanism that recruits RNase H to cleave target mRNA, while siRNA operates through the RISC complex with a double-stranded RNA. AUMsilence is self-delivering without transfection reagents, has fewer off-target effects, and works in difficult-to-transfect cells.

What diseases can be targeted with AUM Biotech products?

AUM Biotech products can target a wide range of diseases including cancer, neurodegenerative diseases like Alzheimer's and Parkinson's, viral infections, immunological disorders, genetic disorders, and rare diseases. The technology has been validated in studies on tauopathies, liver cancer, and other conditions.

How does AUMsilence compare to CRISPR for gene silencing?

Unlike CRISPR which permanently edits the genome, AUMsilence provides temporary and reversible gene silencing at the mRNA level. AUMsilence is simpler to use (just add to cell culture), requires no viral vectors or plasmids, has a faster workflow (days vs. weeks), and avoids potential off-target genomic modifications.

Does AUM Biotech use FANA technology in its products?

AUM Biotech's products incorporate advanced chemical modifications including 2′-deoxy-2′-fluoro-D-arabinonucleic acid (FANA) technology. FANA ASOs and FANA oligos provide enhanced stability, improved target binding, and increased nuclease resistance compared to traditional oligonucleotides. This technology contributes to the high efficiency and self-delivering capabilities of AUM Biotech's products.

Can AUM Biotech products be used in difficult-to-transfect cells?

Yes, AUM Biotech's self-delivering antisense oligonucleotides (sdASO™) are specifically designed to work in difficult-to-transfect cells where traditional methods like lipofection, electroporation, or viral vectors often fail. They have been successfully used in primary neurons, macrophages, T cells, B cells, dendritic cells, cardiomyocytes, hepatocytes, stem cells, and other challenging cell types. The technology eliminates the need for transfection reagents like Lipofectamine or RNAi MAX, making it ideal for gene knockdown studies in these hard-to-transfect cells.

What are the advantages of AUM Biotech's technology over traditional transfection methods?

AUM Biotech's self-delivering antisense oligonucleotides offer several advantages over traditional transfection methods: 1) No need for transfection reagents like Lipofectamine or RNAi MAX, eliminating reagent costs and toxicity; 2) Higher efficiency in difficult-to-transfect cells including primary neurons, macrophages, T cells, and stem cells; 3) Simplified protocols with just a single-step 'add and forget' approach; 4) Reduced cellular stress and toxicity compared to lipofection or electroporation; 5) Consistent results across different cell types and experimental conditions; 6) Effective in both in vitro and in vivo applications without special delivery vehicles.

What cell types work best with AUM Biotech's gene silencing technology?

AUM Biotech's self-delivering antisense oligonucleotides work exceptionally well in a wide range of cell types, but they particularly excel in difficult-to-transfect cells where traditional methods struggle. These include primary neurons, macrophages, T cells, B cells, dendritic cells, cardiomyocytes, hepatocytes, stem cells, iPSCs, hematopoietic cells, NK cells, monocytes, lymphocytes, astrocytes, microglia, and endothelial cells. The technology has been validated in both adherent and suspension cells, primary cell cultures, and in vivo models, making it versatile for virtually any experimental system.

What are the key advantages of AUM technology compared to siRNA?

AUM Biotech's sdASO technology offers several key advantages over siRNA: 1) No transfection reagents required - unlike siRNA which typically requires Lipofectamine or other delivery vehicles; 2) Works in difficult-to-transfect cells that are resistant to siRNA approaches; 3) Self-delivering mechanism with gymnotic uptake that eliminates toxicity associated with transfection reagents; 4) Simpler experimental workflow - just add to media with no complex protocols; 5) Higher reproducibility across different cell types; 6) Ability to target nuclear and cytoplasmic RNAs, while siRNA is limited primarily to cytoplasmic targeting; 7) Fewer off-target effects compared to siRNA; and 8) More cost-effective due to elimination of transfection reagents and higher success rates.

Why choose AUM technology over CRISPR for gene silencing?

While CRISPR is powerful for permanent genomic modifications, AUM Biotech's technology offers distinct advantages for many gene silencing applications: 1) No PAM sequence requirements unlike CRISPR-Cas9; 2) Reversible and temporary silencing, providing more experimental flexibility; 3) Much faster workflow - results in days versus weeks for CRISPR; 4) No risk of permanent off-target genomic modifications; 5) No need for clonal selection or stable cell line generation; 6) Significantly simpler protocol with no need for guide RNA design, Cas9 optimization, or donor template creation; 7) Works efficiently in post-mitotic cells where CRISPR often struggles; 8) Easily adjustable dosing for titration experiments; 9) No viral vectors required; and 10) Compatible with transient experiments where permanent modification is undesirable.

Revolutionizing Gene Silencing with Antisense Technology

Self-delivering antisense technology for unprecedented gene silencing precision and ease

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Advancing Biomedical Research through Innovation

AUM Biotech's revolutionary nucleic acids platform for gene silencing and regulation

Revolutionary Technology

AUMsilence platform employs next-generation chemical modifications and AI-driven design to achieve remarkable stability and activity, effectively silencing genes with unprecedented precision.

Self-Delivering

sdASO™ reagents are self-delivering — simply add them to cell culture or inject them in vivo without transfection reagents, electroporation, or viral vectors.

Broad Compatibility

Works across a wide range of cell types and organisms, from cell lines to primary patient cells and animal models, achieving consistent gene knockdown where other methods fail.

Key Advantages Over RNAi and Gene-Editing

AUM BioTech's platform offers multiple advantages that set it apart from siRNA, shRNA, and CRISPR-based methods

Transfection-Free Delivery

sdASO™ reagents are self-delivering — you can simply add them to cell culture or inject them in vivo without the need for lipid transfection reagents, electroporation, or viral vectors.

Broad Compatibility

AUM BioTech's sdASO™ products work across a wide range of cell types and organisms, from cell lines to primary patient cells and animal models, achieving consistent gene knockdown.

High Specificity & Low Off-Target Effects

AUMsilence antisense oligos are designed for maximum sequence specificity, drastically reducing off-target gene effects for cleaner results and more reliable data.

Safety and Non-Toxicity

Because no transfection agents or viral vehicles are required, cellular stress is minimized with no overt toxicity or immune activation in cells and animals.

Rapid, Scalable, and Reversible

Gene silencing is as simple as adding a reagent — no need to construct plasmids or engineer cell lines, saving weeks compared to CRISPR or shRNA workflows.

In-Depth Control and Versatility

Target mRNAs, inhibit microRNAs, or silence lncRNAs using one unified technology for functional genomics, target discovery, and validation.

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AUM BioTech's RNA Silencing Products

Specialized solutions for every RNA silencing need

Self-delivering Antisense Oligonucleotides (sdASO™)

AUM BioTech's premium products that enter cells without transfection reagents

AUMsilence™

mRNA Knockdown Oligos

Specialized antisense molecules designed for potent mRNA silencing in virtually any cell type, with up to 95% knockdown efficiency.

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AUMantagomir™

miRNA Inhibition Oligos

Tailored for microRNA inhibition, allowing researchers to block specific miRNAs and reveal their biological functions or therapeutic potential.

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AUMlnc™

lncRNA Knockdown Oligos

Specifically optimized to bind and degrade lncRNA transcripts, including nuclear-retained RNAs beyond the reach of traditional RNAi.

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AUMblock™

Blocker Oligos

Steric-blocking antisense molecules designed to bind target RNAs and modulate their function without inducing RNA cleavage.

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AUMskip™

Exon Skipping ASOs

Specialized antisense oligonucleotides designed to bind pre-mRNA and redirect splicing, typically by causing a specific exon to be skipped during mRNA processing.

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AUMsilence V+™

Viral RNA Knockdown Oligos

Dedicated solution for viral RNA silencing, accelerating virology research and antiviral therapeutic discovery.

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Transfection-Optimized ASOs (toASO™)

Budget-friendly options for cost-effective research

AUMsaver™

Cost-Effective ASOs

Budget-friendly gene silencing ASOs optimized for standard transfection protocols, offering high potency and specificity at an accessible price point for high-throughput screening and routine lab work.

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Experience the AUM BioTech Difference

AUM BioTech's technology is validated in numerous high-impact publications and successful research projects worldwide

The impact of AUM BioTech's RNA silencing technology is already evident in numerous high-impact publications and successful research projects worldwide. By choosing AUM BioTech as your gene silencing partner, you join a growing community of scientists achieving breakthroughs in areas like:

Cancer therapy research
Neurodegenerative disease mechanisms
Viral infection studies
Immunological research
Stem cell and developmental biology

AUM BioTech's platform not only accelerates discovery but also enhances experimental reproducibility — simpler protocols mean fewer variables and more consistent results across replicates.

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Join the RNA Revolution with AUM BioTech

Ready to elevate your research to the next level? Explore our product pages to find the right gene-silencing solution for your needs, or contact our scientific team for a consultation on how AUM's technology can advance your specific project.

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