Key Advantages Over RNAi and Gene-Editing
Discover why AUM BioTech's RNA silencing platform outperforms siRNA, shRNA, and CRISPR-based methods
AUMsilence sdASO™ RNA silencing technology offers numerous advantages that empower researchers across various disciplines. Our platform combines innovative chemistry, AI-driven design, and self-delivering capabilities to provide best-in-class RNA-silencing products. Whether you're working in vitro or in vivo, with cancer cells or neurons, our sdASO™ products are designed to achieve superior results with unprecedented ease.
Transfection-Free Delivery
AUM BioTech's sdASO™ Enter Cells Without The Need of Transfection Reagents
Unlike siRNAs and other gene silencing technologies, AUM BioTech's AUMsilence self-delivering antisense oligonucleotides (sdASO™) – including AUMsilence, AUMantagomir, AUMlnc, AUMsilence V+, AUMblock, and AUMskip – can enter cells on their own, without any delivery vehicles. This revolutionary capability eliminates one of the biggest challenges in gene silencing research.
Add and Knockdown Approach
Simply add sdASO™ to cell culture or inject them in vivo – no need for lipid transfection reagents, electroporation, or viral vectors. This dramatically simplifies your workflow and saves both time and resources.
Preserve Cell Health
Transfection reagents often cause cellular stress, toxicity, and off-target effects. By eliminating these delivery vehicles, sdASO™ maintain cell viability and provide cleaner results, especially in sensitive cell types.
Reduce Experimental Variables
Transfection efficiency is a major source of variation in gene silencing experiments. AUM BioTech's self-delivering technology removes this variable, leading to more consistent and reproducible results across replicates and between different labs.
Broad Compatibility
Successful Knockdown in Challenging Models
One of the most significant advantages of AUMsilence sdASO™ technology is its effectiveness in cell types and biological systems where other methods often fail. This opens up entirely new research possibilities in fields like immunology, neuroscience, and developmental biology.
Hard-to-Transfect Cells
AUMsilence sdASO™ products show excellent uptake in notoriously difficult cell types like primary immune cells (T cells, B cells, macrophages), neurons, stem cells, and suspension cell lines. This gives you access to more physiologically relevant models for your research.
In Vivo Performance
Unlike siRNAs that require complex delivery formulations, AUMsilence sdASO™ products can be administered systemically or locally in animal models with minimal formulation. They have demonstrated successful gene silencing in mice, rats, and zebrafish embryos when administered through various routes.
3D Cultures & Tissues
AUMsilence sdASO™ products can penetrate 3D cell cultures, organoids, and tissue explants where transfection reagents often fail due to poor penetration. This allows for gene silencing studies in more complex, tissue-like environments that better mimic in vivo conditions.
High Specificity & Low Off-Target Effects
AUMsilence platform is designed to provide maximum targeting precision. Using advanced chemistry and AI-driven design, we ensure specific binding to target RNAs with minimal off-target effects.
No RISC-Associated Off-Targets
Unlike siRNAs, which often cause off-target effects through the RNA-induced silencing complex (RISC) mechanism, AUMsilence ASOs work independently of RISC. This eliminates seed region-based off-target effects that commonly plague RNAi approaches.
AI-Optimized Design
We employ artificial intelligence to design ASO sequences with optimal target binding properties. AUM BioTech's algorithms analyze RNA structure, accessibility, and sequence uniqueness to create ASOs with maximum efficacy and specificity for your target of interest.
Cleaner Data, Confident Results
High specificity means that phenotypic changes you observe after knockdown can be confidently attributed to your target gene. This is crucial for publication-quality data and for making reliable conclusions about gene function in your research.
Safety and Non-Toxicity
AUMsilence ASOs are engineered to maintain high cell viability while delivering potent knockdown. This balance of efficacy and safety makes them ideal for sensitive experiments and translational research.
Non-Toxic at Effective Concentrations
AUMsilence ASOs show minimal toxicity at doses required for efficient knockdown. By avoiding the need for transfection reagents (which can be toxic themselves), AUMsilence sdASO™ maintain cell health and reduce experimental artifacts.
Potent Knockdown
AUMsilence ASOs aim to achieve 75-95% knockdown of target genes. This high level of efficacy means you can observe clear phenotypic effects, even for genes where substantial reduction is needed to see a biological response.
No Immune Activation
AUMsilence platform includes chemical modifications that help avoid triggering innate immune responses. This is particularly important when working with immune cells or in vivo models, where immune activation could confound your results.
In-Depth Control and Versatility
AUMsilence unified platform gives you unprecedented flexibility in how, when, and where you silence genes.
Target Any RNA Class
sdASO™ product line can effectively target mRNAs (AUMsilence), microRNAs (AUMantagomir), and long non-coding RNAs (AUMlnc), allowing you to explore all aspects of RNA biology with a single technology platform. We also offer specialized products for viral RNA targeting (AUMsilence V+), RNA blocking (AUMblock), and splice modulation (AUMskip).
Nuclear & Cytoplasmic Activity
Unlike siRNAs which primarily function in the cytoplasm, AUMsilence sdASO™ can target RNAs in both nuclear and cytoplasmic compartments. This means you can effectively silence nuclear-retained lncRNAs and pre-mRNAs, expanding your experimental possibilities.
Rapid, Scalable, and Reversible
Gene silencing with sdASO™ is fast (typically showing effects within 24-48 hours), scales easily to high-throughput experiments, and is reversible – allowing for temporal studies of gene function without permanent genetic modifications.
Advanced RNA Manipulation
Beyond Gene Silencing
Our expanded sdASO™ portfolio includes specialized tools for advanced RNA manipulation beyond traditional knockdown. With AUMblock™ and AUMskip™, researchers can now precisely modulate RNA function and processing with the same self-delivering convenience.
Steric Blocking with AUMblock™
AUMblock™ is designed to bind RNA without triggering degradation, instead physically blocking interactions with proteins or other RNAs. This steric hindrance approach enables researchers to selectively inhibit RNA function without reducing its abundance—ideal for studying RNA-protein interactions, blocking miRNA binding sites, or preventing ribosome association.
Splice Modulation with AUMskip™
AUMskip™ targets pre-mRNA splice junctions to modulate alternative splicing patterns. By blocking specific splice sites, these self-delivering ASOs can induce exon skipping or inclusion, allowing researchers to generate therapeutic protein variants or study the function of specific isoforms in cellular and animal models.
Comprehensive RNA Biology Toolkit
Using AUMsilence sdASO™ portfolio (AUMsilence™, AUMantagomir™, AUMlnc™, AUMsilence V+™, AUMblock™, and AUMskip™), researchers can now address virtually any question in RNA biology—from degradation to functional modulation to splice regulation—all with the advantage of self-delivery and AI-optimized design.
Side-by-Side Comparison
See how AUM BioTech's sdASO™ technology compares to traditional gene silencing methods
| Feature | AUM sdASO™ | siRNA/RNAi | shRNA | CRISPR/Cas9 |
|---|---|---|---|---|
| Delivery | Self-delivering; no transfection needed | Requires transfection reagents or special delivery vehicles | Requires viral vectors or plasmid transfection | Requires delivery as DNA, RNA, or protein complex |
| Target Location | Nuclear & cytoplasmic RNA | Primarily cytoplasmic RNA | Primarily cytoplasmic RNA | DNA (genome) |
| Onset of Effect | Rapid (24-48 hours) | Rapid (24-48 hours) | Slower (days to weeks for expression) | Slow (days to weeks for editing & clonal isolation) |
| Duration | Transient, controllable (re-dosing possible) | Transient (days) | Long-term if integrated | Permanent (DNA change) |
| Off-target Effects | Minimal (no RISC-based off-targets) | Significant (seed-based off-targets common) | Similar to siRNA plus integration effects | Potential off-target genome edits |
| Hard-to-transfect Cells | Excellent performance | Poor performance | Limited by viral transduction | Variable, often difficult |
| Setup Complexity | Very simple (add to medium) | Moderate (optimize transfection) | Complex (cloning, virus, selection) | Very complex (design, cloning, selection) |
| Time to Results | Days | Days | Weeks | Weeks to months |
| RNA Manipulation Options | Complete: knockdown, blocking, splice modulation | Primarily knockdown only | Primarily knockdown only | DNA level only (knockouts, mutations) |
Case Study: Immunology Research
A research team studying T cell differentiation needed to silence a key transcription factor in primary human T cells:
- Traditional siRNA approaches failed due to poor transfection of primary T cells and high toxicity with electroporation
- AUMsilence sdASO™ achieved >75% knockdown by simply adding to activated T cell cultures
- No toxicity was observed, and cells maintained normal proliferation and activation markers
- The simplicity allowed them to test 5 different transcription factors in parallel, accelerating their research timeline by months
Case Study: Splice Modulation for Disease Model
Researchers investigating a genetic disorder needed to induce exon skipping in primary neurons:
- AUMskip™ sdASO™ was designed to target a specific exon-intron junction
- The self-delivering capability allowed direct application to sensitive neuronal cultures without transfection toxicity
- RT-PCR confirmed successful exon skipping in >70% of transcripts
- The resulting protein isoform corrected the disease phenotype in the neuron model, providing proof-of-concept for a therapeutic approach
Ready to Experience the Difference?
AUM Biotech's AI-powered AUMsilence platform and self-delivering antisense technology (sdASO™) can transform your research by providing efficient, specific gene silencing and RNA modulation in even the most challenging experimental systems. From simple cell culture to complex in vivo models, AUM BioTech's technology delivers results where others fail.